Monday, September 18th, 2023, at 3:00 pm and Tuesday, September 19th, at 9.00 am
This year we have 5 tutorials:
- 1) Flow Cytometry, Monday, 3:00 pm
- 2) Image Analysis, Monday, 3:00 pm
- 3) Mass Cytometry, Monday, 3:00 pm
- 4) Mechanocytometry for label-free cell sorting, Tuesday, 9.00 am
- 5) Publication, data integrity, FAIR data, Tuesday, 9.00 am
The abstracts of the individual tutorials will be available here shortly. Check back again!
1) Cycle Analysis by Flow Cytometry:
Monday, September 18th at 3:00 pm
Chairs: Claudia Giesecke-Thiel & Toralf Kaiser
We will briefly cover some basics of flow cytometry and then move directly to the details of cell cycle analysis using flow cytometry. We review the fundamentals in this field so that you are well prepared to conduct your own experiments. In this context, we will also introduce new technologies, in particular MAPS-FC (multi-angle pulse shape flow cytometry), an approach that measures angle- and time-resolved scattered light for high-throughput cell characterization to circumvent the constraints of conventional flow cytometry, developed at the DRFZ (Kage et al. 2021) and pulse shape-activated cell sorting (PACS), with a focus on cell cycle analysis without the need for fluorescent labeling.
4) Mechanocytometry for label-free sorting:
Tuesday, September 19th at 9:00 am
Chair: Oliver Otto
Assume you are interested in the identification and classification of cells but you lack appropriate fluorescence markers or their application is inhibited in translational research. How can you then classify and characterize your cells?
Mechanocytometry can be a solution. It works like this: you excert forces on a cell and observe its deformation. What you uncover are mechanical features of cells, which establish a label-free biomarker on the cellular level instead of the molecular level. Why is this an interesting scientific target? Because the response of a cell under physiological and pathological condition depends on the cytoskeleton, the nucleus, the membrane and also sub-cellular structures. Did you know that neutrophils change their stiffness when being activated, that red blood cells get stiffer when infected with malaria or that human stem cells derived from bone marrow are softer than the ones from peripheral blood?
Real-time deformability cytometry (RT-DC) is the fastest way to do mechanocytometry. RT-DC is a combination of force application for single cells and fast imaging complemented with the capabilities of fluorescence-based flow cytometry. Besides biophysical information (stiffness, shape, morphology…) RT-DC also provides you with a brightfield image of every cell. What you see is what you get! You can easily identify debris or explore outliers. Importantly, mechanocytometry can also be combined with cell isolation, i.e., you can sort cells label-free based on biophysical parameters.
If you are interested in having a different perspective at your samples, register for the tutorial: Mechanocytometry for label-free cell sorting. We will show you live measurements and sorting using the AcCellerator from Zellmechanik Dresden equipped with a SortingModule.